A critical step in the development of non-invasive neuromodulation is the ability to verify target engagement. Such verification remains particularly challenging for deeper brain structures, despite their fundamental contributions to brain function and cognition. Here, we propose to leverage the high spatial resolution and whole-brain coverage of functional magnetic resonance imaging (fMRI). We will image circuit-specific neuromodulation following TUS to both the amygdala and the thalamus. By probing the spatial, temporal, and state specificity of TUS effects using neuroimaging, we aim to validate TUS as a rigorous tool for non-invasive deep brain modulation.